Who is the test for?
For individuals seeking to determine the presence or absence of occult blood in faeces.
How to use

Sample Collection (First, Second, and Third Day):
- Wash hands and collect faeces on a separate paper strip each day.
- Dip the vial tip into the faeces, avoiding excess for accurate results.
- Securely cap the vial, shake to suspend the sample, and store it in the refrigerator.
- Dispose of the paper strip in the toilet.
Test Realization (After 3 Days Collection Period):
- Tear open the pouch, remove the device, and let the vial reach room temperature.
- Break the purple tip, place the device on a flat surface.
- Add precisely five drops of the extract into the sample well.
- Read the result after 10 minutes, avoiding interpretation after 15 minutes.
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Results and recommendations
Analyze
Result
Summery

Two coloured lines appear in the windowunder the marks T (Test) and C (Control). The intensity of theline T may be clearer than the intensity of the line C. Thisresult means that blood is present in the faeces and that youshould consult a doctor.

Only one coloured line appears underthe mark C (Control). This result means that no blood isdetectable in the faeces sample.

No line appears under the mark C. Inthis case, it is not possible to interpret the test, which mustbe considered as non valid. It is recommended to repeatthe test with a new Poly-Check® test and a fresh sample offaeces.
Questions and answers
Gastro-intestinal lesions are often bleeding. The blood can be detected specifically by Poly-Check® in the faeces when concentration is higher than 10 ng/mL. The presence of blood can be explained by several causes: ulcers, haemorrhoids, colorectal cancer. The probability for intermittent bleeding to be detected is increased by the procedure of sample collection over 3 running days.
Poly-Check® test uses a pair of antibodies detecting specifically human haemoglobin by producing a coloured test line under the T mark of the cassette. A control line capturing the reagent excess appears as a coloured line under the C mark of the cassette. In case both lines appear, the test indicates that the level of haemoglobin is higher than normal (> 10 ng/mL determined against purified human haemoglobin material).
This procedure allows a more accurate test result for the patient especially for early stages of diseases and, therefore, greater probability to cure them.
Poly-Check® test can be performed at any time of the day when having a bowelmovement.
The results are accurate as far as the instructions are carefully respected.Nevertheless, the result can be falsified if Poly-Check® test gets wet before testperforming or stool collecting steps are not correctly performed or if the sampleis contaminated with blood from other origins than faeces (cuts, haemorrhoids,menses etc…) or if an incorrect number of drops is dispensed in the sample well.
The colour and intensity of the lines have no importance for resultinterpretation. The lines should only be homogenous and full.
When this line appears, it only means that the test performed correctly and that the test result is valid, but it does not play any role in result interpretation.
No. The test should be read after 10 minutes and before 15 minutes after havingadded the extract. The results are reliable up to 15 minutes.
If the result is positive, it means that blood has been detected in the stoolsample and that you should consult a doctor to show the test results. Then, thedoctor will decide what you have to do.
If the result is negative, it means that the test could not detect blood in stoolsample. Meanwhile for the patients aged of 45 years and over or havingrelatives suffering from colorectal cancer, it is recommended to perform a testevery year.
The Poly-Check® test is accurate and has been used for more than 10 years by professionals in the field. Evaluation reports show an overall agreement of at least 93% ([86.78 – 97.17*]) with different reference methods. Although this test is reliable, false positive or false negative results could be obtained.
*CI 95%: 95% confidence interval